Tioluwanimi Obileye | 2026 I.S. Symposium
狈补尘别:听Tioluwanimi Obileye
罢颈迟濒别:听Exploring How Pseudomonas chlororaphis 14B11 Bacteria Responds to Simultaneous Bacteriophage-Antibiotic Therapy鈥攖he Evolution of Double Resistant Strains
Major: Biochemistry and Molecular Biology
惭颈苍辞谤:听French and Francophone Studies
础诲惫颈蝉辞谤:听Stephanie Strand
Antibiotics have long been used to clinically treat bacterial infections, but overuse has led to the emergence of antibiotic-resistant bacteria. Although bacteriophage therapy was put forward as a potential alternative, it can lead to resistance in bacteria. With limited research on bacterial susceptibility to simultaneous bacteriophage-antibiotic treatment, this project investigates the emergence of double (phage-antibiotic) resistant bacteria strains, the presence of mutations in target gene and the mechanism of resistance. We grew Pseudomonas chlororaphis 14B11 under simultaneous exposure to bacteriophage & antibiotic, and we tested the strains for resistance. gDNA of select mutants were amplified and sequenced to identify oprM gene mutations. Only phage-antibiotic resistant strains grew at concentrations above MIC, and they showed no OprM mutations, suggesting that double resistance may arise through intrinsic mechanisms. Overall, our findings indicate the contribution of other genes to the evolution of phage-antibiotic resistant strains.
Posted in Symposium 2026 on May 1, 2026.
