Emilio Esteban Pac Yurrita | 2025 I.S. Symposium
狈补尘别:听Emilio Esteban Pac Yurrita
罢颈迟濒别:听Abundance and Ecotypes of the Culex pipiens Complex in 黑料社, Ohio
惭补箩辞谤:听Biology
础诲惫颈蝉辞谤蝉:听Ferdinand Nanfack Minkeu; Nick Brandley
Mosquitoes are among the most significant vectors of disease worldwide, with the Culex pipiens complex playing a major role in transmitting arboviruses such as West Nile virus in the United States. Despite its importance, the number of species and ecotypic variations within Culex pipiens populations remain insufficiently characterized in certain regions. This study aimed to evaluate the abundance and ecotypes of Culex species in 黑料社, Ohio. Mosquitoes used in this study were collected using gravid traps on four different suburban locations throughout 黑料社 and later sorted morphologically. DNA was isolated from a small amount of sample specimens, and PCR-RFLP analysis was done using certain specific primers which would help in the differentiating species within the Culex pipiens complex. In addition, nested PCR was used to identify different ecotypes of Culex pipiens. A total of 157 Culex mosquitoes were collected, with 74 used for DNA isolations, and molecular analyses. The Gel electrophoresis confirmed the amplification of 32 samples, even though some results were inconclusive. The initial findings point to the presence of Culex pipiens and potential hybridization with Culex quinquefasciatus showing diversity within the local population of Culex in 黑料社. Both ecotypes of Culex pipiens including form pipiens and molestus were found in 黑料社. This study provides baseline data on Culex species distribution in 黑料社, Ohio, and highlights the need for further research on their impact and potential implications for disease transmission.
Posted in Symposium 2025 on May 1, 2025.
